Growth of Neurospora.

Biotransformation of Hydrocortisone by Neurospora crassa

The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...

Growth Responses of a Sulfonamide-requiring Mutant Strain of Neurospora.

Growth inhibition of Neurospora by canavanine, and its reversal.

Canavanine, an amino acid from jack beans, was discovered by Kitagawa and coworkers in 1929 (1, 2). The substance is not combined in the proteins of the seed, but occurs in the free state, and makes up 2.5 per cent of the dry weight of jack beans (3). In a series of papers available to the authors for the most part in abstract only, the Japanese workers have reported extensive investigations in...

Germination and growth of neurospora at low water activities.

When the water activity (a(w)) of the medium is lowered by the addition of NaCl or nonelectrolytes, an inhibition of germination, growth rate, and total growth is observed in Neurospora crassa. Inhibition of conidial germination is separable from the other effects and is caused, in large measure, by the loss from conidia in media of low water activity of a substance that is essential for their ...

Calcium gradient dependence of Neurospora crassa hyphal growth.

A tip-high cytoplasmic calcium gradient has been identified as a requirement for hyphal growth in the fungus Neurospora crassa. The Ca2+ gradient is less steep compared to wall vesicle, wall incorporation and vesicular Ca2+ gradients, but this can be explained by Ca2+ diffusion. Analysis of the relation between the rate of hyphal growth and the spatial distribution of tip-localized calcium indi...